Laser-Assisted Hatching

A covering layer, or 'shell' surrounds embryos or blastocysts called the Zona Pellucida (ZP). The zona has an important role in fertilisation as it allows only one sperm to penetrate the zona and enter the egg to achieve fertilisation.

It also acts to prevent premature implantation in the Fallopian tube and may help prevent the early embryo from being attacked by cells of the immune system. The embryos have to "hatch" or break out of the zona in order to embed into the endometrium lining the uterine cavity. This occurs about four to five days after embryo transfer when the embryo is at the blastocyst stage. Naturally this takes place by expanding/contracting of the zona until it distorts, allowing the blastocyst to "hatch".

Methods of Assisted Hatching in India

Chemical:-This involves chemicals (acid tyrodes) used to produce a weakened area of the zona. The chemical is applied through a microtool, manipulated by an embryologist. It is not entirely accurate, as when working with such small amounts of chemicals, it is hard to quantify the dose with pinpoint accuracy. The acid then has to be washed off in order to prevent further damage to the embryo.

Laser:- The zona is drilled by the microlaser system called Fertilase. It creates a clean-cut precise incision in the zona.

This is more accurate for several reasons : -

The process is fast compared to the chemical method, thus the embryo does not have to be held still by a suction pipette. As well as this factor, we use a vibration free table to avoid any accidental movement of the embryo.
The accuracy of the laser is predetermined by its programming, and is therefore not dependent on the skill of the operator. There is a high degree of reproducible accuracy.

 

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Address: Dr. Indira Ganeshan IVF & Fertility Centre, A-11(Basement),
South Extension Part 2, New Delhi, India, Pincode-110049

 
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